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Addgene

pcDNA-FLAG-Co-NF-kB
(Plasmid #180197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-FLAG
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Co-NF-kB
  • Alt name
    Capsaspora NF-kB
  • Species
    Capsaspora owczarzaki
  • Insert Size (bp)
    3672
  • Mutation
    aa 2-1224
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG-tagged fusion protein (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV promoter
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for human cell expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-FLAG-Co-NF-kB was a gift from Thomas Gilmore (Addgene plasmid # 180197 ; http://n2t.net/addgene:180197 ; RRID:Addgene_180197)

  • For your References section:

    Comparison of NF-kappaB from the protists Capsaspora owczarzaki and Acanthoeca spectabilis reveals extensive evolutionary diversification of this transcription factor. Williams LM, Sridhar S, Samaroo J, Peart J, Adindu EK, Addanki A, DiRusso CJ, Aguirre Carrion PJ, Rodriguez-Sastre N, Siggers T, Gilmore TD. Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2. 10.1038/s42003-021-02924-2 PubMed 34916615
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