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pEF/myc/ER FLIPglu-3.2mDelta13V
(Plasmid #18019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF/myc/ER
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5603
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPglu F16A S112A Delta13
  • Alt name
    ECFP-mglB-Venus
  • Alt name
    Periplasmic glucose binding
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    F16A, S112A in binding protein
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF/myc/ER FLIPglu-3.2mDelta13V was a gift from Wolf Frommer (Addgene plasmid # 18019 ; http://n2t.net/addgene:18019 ; RRID:Addgene_18019)

  • For your References section:

    Facilitative plasma membrane transporters function during ER transit. Takanaga H, Frommer WB. FASEB J. 2010 Aug . 24(8):2849-58. 10.1096/fj.09-146472 PubMed 20354141
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