pRB131
(Plasmid #180174)

• Purpose: pAAV construct GluN1A714L (shRNA resistant)
• Depositing Lab: Richard Bergeron
• Publication: Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi: 10.1016/j.isci.2021.103539. eCollection 2022 Jan 21.

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Backbone

• Vector backbone: pAAV-CW3SL-EGFP
• Backbone manufacturer: Addgene#61463
• Modifications to backbone: EGFP dropped out by NheI and EcoRI. EcoRI site was blunt ended and GluN1 cloned in as a NheI EcoRV fragment.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GluN1
• Alt name: GriN1/ NMDAR1
• Species: R. norvegicus (rat)
• Insert Size (bp): 3545
• Mutation: A714L
• Entrez Gene: Grin1 (a.k.a. GluN1, NMDAR1, NR1)
• Tag / Fusion Protein:
  • Phluorin (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: GluN1 SeqF1 CATGGTGTGGGCTGGTTTCG
• 3′ sequencing primer: GluN1 SeqR1 TGCAGGTTCTTCCTCCACAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry