pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G
(Plasmid
#18017)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLIPglu D154A Delta13V ECFP-W67G
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Alt nameECFP-mglB-Venus
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Alt namePeriplasmic glucose binding
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SpeciesE. coli
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Insert Size (bp)2400
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MutationmglB-D154A; non-functional ECFP
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Tags
/ Fusion Proteins
- ECFP-W67G (N terminal on insert)
- Venus (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G was a gift from Wolf Frommer (Addgene plasmid # 18017 ; http://n2t.net/addgene:18017 ; RRID:Addgene_18017) -
For your References section:
Facilitative plasma membrane transporters function during ER transit. Takanaga H, Frommer WB. FASEB J. 2010 Aug . 24(8):2849-58. 10.1096/fj.09-146472 PubMed 20354141
