pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G
(Plasmid #18016)

• Depositing Lab: Wolf Frommer
• Publication: Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLIPglu D154A Delta13V Venus-Y67G
• Alt name: ECFP-mglB-Venus
• Alt name: Periplasmic glucose binding
• Species: E. coli
• Insert Size (bp): 2400
• Mutation: mglB-D154A; non-functional Venus
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • Venus-Y67G (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry