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H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP
(Plasmid #180155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Backbone manufacturer
    Hyung-song Nam, Capecchi laboratory
  • Vector type
    Mammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    The DNA sequence flanked by the loxP sites are prone to deletion in E. coli by recombination. Double-selecting with ampicillin and kanamycin is recommended. The concentrations can be halved if growth is too slow.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    td-sfGFP
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CAG promoter is from pCAGGS obtained from BCCM/LMBP. Neo* is from plasmid #22687. bGH pA is from Neal Copeland. SV40 pA is from Clontech. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. Backbone is from Stratagene.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A reporter expression cassette with short homology arms to Hipp11 on either side. Constructed originally for generating Hipp11 reporter allele targeting vector by recombineering. Untested for other purposes, but may be useful for CRISPR knock-in as well.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP was a gift from Mario Capecchi (Addgene plasmid # 180155 ; http://n2t.net/addgene:180155 ; RRID:Addgene_180155)
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