R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
(Plasmid #180152)

• Purpose: tdTomato cre reporter with Rosa26 recombineering homology arms
• Depositing Lab: Mario Capecchi
• Publication: Mouse safe harbor locus targeting plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Growth instructions: The DNA sequence flanked by the loxP sites are prone to deletion in E. coli by recombination. Double-selecting with ampicillin and kanamycin is recommended. The concentrations can be halved if growth is too slow.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • ** 5 CAG-5' loxP - FNF -3xpA-3' loxP-tdTomato-WPRE-bpA.gb
• A portion of this plasmid was derived from a plasmid made by: CAG promoter is from pCAGGS obtained from BCCM/LMBP. Neo* is from plasmid #22687. bGH pA is from Neal Copeland. SV40 pA is from Clontech. tdTomato is from Roger Tsien. WPRE is from Miguel Ramalho-Santos. Backbone is from Stratagene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A reporter expression cassette with short homology arms to Rosa26 on either side. Constructed originally for generating Rosa26 reporter allele targeting vector by recombineering. Untested for other purposes, but may be useful for CRISPR knock-in as well.