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Addgene

pCS2-Bighead S-FC
(Plasmid #180147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4906
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bighead.S
  • Species
    X. laevis (frog)
  • Tag / Fusion Protein
    • FC

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-Bighead S-FC was a gift from Edward De Robertis (Addgene plasmid # 180147 ; http://n2t.net/addgene:180147 ; RRID:Addgene_180147)

  • For your References section:

    Bighead is a Wnt antagonist secreted by the Xenopus Spemann organizer that promotes Lrp6 endocytosis. Ding Y, Colozza G, Sosa EA, Moriyama Y, Rundle S, Salwinski L, De Robertis EM. Proc Natl Acad Sci U S A. 2018 Sep 25;115(39):E9135-E9144. doi: 10.1073/pnas.1812117115. Epub 2018 Sep 12. 10.1073/pnas.1812117115 PubMed 30209221
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