pCS2-hFAM3B-FLAG
(Plasmid
#180146)
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PurposeCoding sequence of human FAM3B in pCS2 with C terminal FLAG tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFAM3B
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Alt namePANDER
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SpeciesH. sapiens (human)
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Entrez GeneFAM3B (a.k.a. 2-21, C21orf11, C21orf76, ORF9, PANDER, PRED44)
- Promoter SP6
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-hFAM3B-FLAG was a gift from Edward De Robertis (Addgene plasmid # 180146 ; http://n2t.net/addgene:180146 ; RRID:Addgene_180146) -
For your References section:
The cytokine FAM3B/PANDER is an FGFR ligand that promotes posterior development in Xenopus. Zhang F, Zhu X, Wang P, He Q, Huang H, Zheng T, Li Y, Jia H, Xu L, Zhao H, Colozza G, Tao Q, De Robertis EM, Ding Y. Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2100342118. doi: 10.1073/pnas.2100342118. 10.1073/pnas.2100342118 PubMed 33975953