pCS2-LRP6-3XFlag
(Plasmid
#180144)
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Purposeflag tagged LRP6
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 180144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman LRP6
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SpeciesH. sapiens (human)
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Entrez GeneLRP6 (a.k.a. ADCAD2, STHAG7)
- Promoter SP6
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Tag
/ Fusion Protein
- FLAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-LRP6-3XFlag was a gift from Edward De Robertis (Addgene plasmid # 180144 ; http://n2t.net/addgene:180144 ; RRID:Addgene_180144) -
For your References section:
Wnt-inducible Lrp6-APEX2 interacting proteins identify ESCRT machinery and Trk-fused gene as components of the Wnt signaling pathway. Colozza G, Jami-Alahmadi Y, Dsouza A, Tejeda-Munoz N, Albrecht LV, Sosa EA, Wohlschlegel JA, De Robertis EM. Sci Rep. 2020 Dec 9;10(1):21555. doi: 10.1038/s41598-020-78019-5. 10.1038/s41598-020-78019-5 PubMed 33299006