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Addgene

Anti-Kv2.1 K+ channel (External) [K39/25R]
(Antibody #180104)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 180104-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 180104-rAb.T Limited Stock Available, 4 units left
20 µg of purified recombinant antibody
$85 *

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Features

Production & Usage

  • Production Plasmid
    Anti-Kv2.1 K+ channel (External) [K39/25R]
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2750663

Terms and Licenses

Target Antigen

Protein Potassium voltage-gated channel subfamily B member 1
Antigen Description Amino acids 211–229 of human Kv2.1
Antigen Amino Acid Sequence
LPELQSLDEFGQSTDNPQL
Species H. sapiens (human)
Additional Species Reactivity M. musculus (mouse),  R. norvegicus (rat)
Gene KCNB1
Alternative Names
  • Delayed rectifier potassium channel 1
  • DRK1
  • h-DRK1
  • Voltage-gated potassium channel subunit Kv2.1
External References

Applications (3)

Immunocytochemistry

1 image
Immunocytochemistry image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass

Western Blot

1 image
Western Blot image by James Trimmer, UC Davis
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Recombinant
Sample Species
R. norvegicus (rat)
Result
Pass
1 image
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass

Addgene Comments

Although this antibody has a high degree of background staining in western blot, we are making it available because it is one of the few antibodies that recognizes the extracellular domain of the Kv2.1 K+ channel and we understand that it is useful to scientists.
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Kv2.1 K+ channel (External) [K39/25R] - from James Trimmer (Addgene antibody # 180104 ; http://n2t.net/addgene:180104 ; RRID:AB_2750663)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360