pDON223 Spike L241-L242-A243-
(Plasmid
#180070)
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PurposeSars-CoV-2 protein mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDON223
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 Spike L241-L242-A243-
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SpeciesSARS-CoV-2
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MutationSpike L241-L242-A243-
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
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Tag
/ Fusion Protein
- MAC-tag (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDON223 Spike L241-L242-A243- was a gift from Markku Varjosalo (Addgene plasmid # 180070 ; http://n2t.net/addgene:180070 ; RRID:Addgene_180070) -
For your References section:
SARS-CoV-2-host proteome interactions for antiviral drug discovery. Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S, Vartiainen MK, Pietiainen V, Poso A, Varjosalo M. Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396. 10.15252/msb.202110396 PubMed 34709727