pT3-EF1aH-PIK3CA H1047R
(Plasmid #180031)

• Purpose: This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
• Depositing Lab: Xin Chen
• Publication: Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv.13055.

Backbone

• Vector backbone: pT3-EF1aH
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PIK3CA H1047R
• Alt name: phosphoinositide 3-kinase
• Alt name: PI3K
• Alt name: p110 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 3207
• Mutation: H1047R
• GenBank ID: NM_006218.4
• Entrez Gene: PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
• Promoter: EF1a
• Tag / Fusion Protein:
  • HA tag (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF1a-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HA-PIK3CAH1047R was cloned from Addgene #12524: pBabe-HA-PIK3CAH1047R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry