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Addgene

GB1-ALIX(P801G)
(Plasmid #180025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone size w/o insert (bp) 5644
  • Total vector size (bp) 8533
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GB1-His6-TEV-ALIX(1-868, P801G)
  • Alt name
    ALIX
  • Alt name
    PDCD6IP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2889
  • Mutation
    P801G
  • Entrez Gene
    PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
  • Tag / Fusion Protein
    • GB1-His6-TEV (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB1-ALIX(P801G) was a gift from Lalit Deshmukh (Addgene plasmid # 180025 ; http://n2t.net/addgene:180025 ; RRID:Addgene_180025)

  • For your References section:

    Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation. Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L. Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. Epub 2023 Jul 14. 10.1126/sciadv.adg3913 PubMed 37450591
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