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pUASp-Egl-Trbo
(Plasmid #179993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASp-attB-K10
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 14000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Egl-Trbo
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    4053
  • Promoter UAS-Gal4
  • Tag / Fusion Protein
    • TurboID (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer GCACGTTTGCTTGTTGAGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASp-Egl-Trbo was a gift from Graydon Gonsalvez (Addgene plasmid # 179993 ; http://n2t.net/addgene:179993 ; RRID:Addgene_179993)

  • For your References section:

    In vivo proximity biotin ligation identifies the interactome of Egalitarian, a Dynein cargo adaptor. Baker FC, Neiswender H, Veeranan-Karmegam R, Gonsalvez GB. Development. 2021 Nov 15;148(22). pii: 273472. doi: 10.1242/dev.199935. Epub 2021 Nov 18. 10.1242/dev.199935 PubMed 35020877
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