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Addgene

HEpl016
(Plasmid #179991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px330
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SiCas12i
  • Alt name
    humanized
  • Species
    Synthetic
  • Insert Size (bp)
    3411
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HEpl016 was a gift from Yingsi Zhou (Addgene plasmid # 179991 ; http://n2t.net/addgene:179991 ; RRID:Addgene_179991)

  • For your References section:

    An engineered xCas12i with high activity, high specificity, and broad PAM range. Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, Li J, Zhong N, Zhang M, Zhou Y, Yang H. Protein Cell. 2023 Jun 28;14(7):538-543. doi: 10.1093/procel/pwac052. 10.1093/procel/pwac052 PubMed 37378659
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