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pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tag
(Plasmid #179974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Nsp7
  • Alt name
    RaTG13 Nsp7
  • Alt name
    SARS-CoV-2 Nsp7 and RaTG13 Nsp7 have the same amino acid sequence and codon optimized sequence.
  • Species
    Synthetic
  • Mutation
    human codon-optimized
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tag was a gift from Konstantin Sparrer (Addgene plasmid # 179974 ; http://n2t.net/addgene:179974 ; RRID:Addgene_179974)

  • For your References section:

    Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities. Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, Christensen MH, Conzelmann C, Muller JA, Srinivasachar Badarinarayan S, Sturzel CM, Forne I, Stenger S, Conzelmann KK, Munch J, Schmidt FI, Sauter D, Imhof A, Kirchhoff F, Sparrer KMJ. Cell Rep. 2021 May 18;35(7):109126. 10.1016/j.celrep.2021.109126 PubMed 33974846
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