CMV-HA-hTAF1
(Plasmid #17997)

• Depositing Lab: Robert Tjian
• Publication: Ruppert et al Nature. 1993 Mar 11. 362(6416):175-9.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4097
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hTAF1
• Alt name: hTAFII250
• Species: H. sapiens (human)
• Insert Size (bp): 6000
• Entrez Gene: TAF1 (a.k.a. BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, MRXS33, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: StuI (destroyed during cloning)
• 5′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for more information. A BamHI/KpnI fragment from pHAX250 (pCMVHAX hTAFII250) was cloned into BamHI/StuI cut pCS2+.