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Addgene

pCG_SARS-CoV1 C-V5-tag ORF3a
(Plasmid #179969)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 179969 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-1 ORF3a
  • Species
    Synthetic
  • Mutation
    human codon-optimized
  • Entrez Gene
    ORF3a (a.k.a. sars3a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCG_SARS-CoV1 C-V5-tag ORF3a was a gift from Konstantin Sparrer (Addgene plasmid # 179969 ; http://n2t.net/addgene:179969 ; RRID:Addgene_179969)

  • For your References section:

    Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities. Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, Christensen MH, Conzelmann C, Muller JA, Srinivasachar Badarinarayan S, Sturzel CM, Forne I, Stenger S, Conzelmann KK, Munch J, Schmidt FI, Sauter D, Imhof A, Kirchhoff F, Sparrer KMJ. Cell Rep. 2021 May 18;35(7):109126. 10.1016/j.celrep.2021.109126 PubMed 33974846
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