YFVdelCME-mVenus
(Plasmid
#179953)
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PurposepCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCCI
- Backbone size w/o insert (bp) 8888
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Vector typetransneuronal tracing
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)TransforMax EPI300
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameYFV-17D lacking C, prM, E proteins but with mVenus
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SpeciesYellow fever virus
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Insert Size (bp)9763
- Promoter SP6
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThis plasmid was constructed in collaboration with Dr. John W Schoggins.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Growth conditions and plasmid amplification see attached protocol.
Please note there is a single mutation K206A i in mVenus. Depositor confirms this does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFVdelCME-mVenus was a gift from Wei Xu (Addgene plasmid # 179953 ; http://n2t.net/addgene:179953 ; RRID:Addgene_179953) -
For your References section:
Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D. Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W. Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 10.1038/s41592-021-01319-9. 10.1038/s41592-021-01319-9 PubMed 34824475
