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Addgene

YFVdelNS1-Cre
(Plasmid #179952)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 179952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCCI
  • Backbone size w/o insert (bp) 8888
  • Vector type
    transneuronal tracing

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TransforMax EPI300
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    YFV-17D lacking nonstructural protein 1 (NS1), but with Cre
  • Species
    Yellow fever virus
  • Insert Size (bp)
    11839
  • Promoter SP6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Growth conditions and plasmid amplification see attached protocol

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFVdelNS1-Cre was a gift from Wei Xu (Addgene plasmid # 179952 ; http://n2t.net/addgene:179952 ; RRID:Addgene_179952)

  • For your References section:

    Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D. Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W. Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 10.1038/s41592-021-01319-9. 10.1038/s41592-021-01319-9 PubMed 34824475
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