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pCOA-Leu-FvPPT1
(Plasmid #179923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179923 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pESC-LEU
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 8660
  • Total vector size (bp) 9539
  • Modifications to backbone
    Added the Yarrowia lipolytica autosomal replication sequence ARS68. Substituted the bidirectional promoter Gal1/10 with Yarrowia lipolytica P(EXP1) (fused to PPT1) and P(TEF-1α) for constitutive expression in Saccharomyces cerevisiae
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FvPPT1
  • Alt name
    PPT1
  • Alt name
    PPTase
  • Species
    Fusarium verticillioides
  • Insert Size (bp)
    879
  • Mutation
    Codon optimized for expression in Saccharomyces cerevisiae
  • GenBank ID
    FVEG_01894 XP_018744954
  • Promoter Yarrowia lipolytica EXP1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCOA-Leu-FvPPT1 was a gift from Jens Laurids Sørensen (Addgene plasmid # 179923 ; http://n2t.net/addgene:179923 ; RRID:Addgene_179923)

  • For your References section:

    Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students. Bejenari M, Nielsen L, Spedtsberg EML, Nielsen MR, Pedersen TB, Sorensen JL. J Microbiol Biol Educ. 2023 Nov 27;24(3):e00242-22. doi: 10.1128/jmbe.00242-22. eCollection 2023 Dec. 10.1128/jmbe.00242-22 PubMed 38108002
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