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pTwist-SARS-CoV-2 Δ18 B.1.1.529
(Plasmid #179907)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179907 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist-CMV-BetaGlobin-WPRE-Neo
  • Backbone manufacturer
    Twist
  • Backbone size w/o insert (bp) 6694
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3759
  • Mutation
    A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212; Ins214EPE; G339D; S371L; S373P; S375F; K417N; N440K; G446S; S477N; T478K; E484A; Q493R; G496S; Q498R; N501Y; Y505H; T547K; D614G; H655Y; N679K; P681H; N764K; D796Y; N856K; Q954H; N969K; L981F
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CAG

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Balazs lab Clone 18

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist-SARS-CoV-2 Δ18 B.1.1.529 was a gift from Alejandro Balazs (Addgene plasmid # 179907 ; http://n2t.net/addgene:179907 ; RRID:Addgene_179907)

  • For your References section:

    mRNA-based COVID-19 vaccine boosters induce neutralizing immunity against SARS-CoV-2 Omicron variant. Garcia-Beltran WF, St Denis KJ, Hoelzemer A, Lam EC, Nitido AD, Sheehan ML, Berrios C, Ofoman O, Chang CC, Hauser BM, Feldman J, Roederer AL, Gregory DJ, Poznansky MC, Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB. Cell. 2022 Jan 6. pii: S0092-8674(21)01496-3. doi: 10.1016/j.cell.2021.12.033. 10.1016/j.cell.2021.12.033 PubMed 34995482
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