Skip to main content
Addgene

pEGFP-SF2
(Plasmid #17990)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SF2
  • Alt name
    ASF
  • Species
    H. sapiens (human)
  • Mutation
    contains SF2 (aa1-210)
  • Entrez Gene
    SRSF1 (a.k.a. ASF, NEDFBA, SF2, SF2p33, SFRS1, SRp30a)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-SF2 was a gift from Tom Misteli (Addgene plasmid # 17990 ; http://n2t.net/addgene:17990 ; RRID:Addgene_17990)

  • For your References section:

    High mobility of proteins in the mammalian cell nucleus. Phair RD, Misteli T. Nature. 2000 Apr 6. 404(6778):604-9. 10.1038/35007077 PubMed 10766243
Related items:
Commonly requested with: