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Addgene

WGA-D
(Plasmid #17989)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    probably pBKS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wheat germ agglutinin isolectin D
  • Alt name
    WGA-D
  • Species
    Triticum aestivum (hexaploid wheat)
  • Insert Size (bp)
    998
  • Mutation
    N/A
  • GenBank ID
    M25537

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13F or M13R
  • 3′ sequencing primer M13F or M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WGA-D was a gift from Natasha Raikhel (Addgene plasmid # 17989 ; http://n2t.net/addgene:17989 ; RRID:Addgene_17989)

  • For your References section:

    Nucleotide sequences of cDNA clones encoding wheat germ agglutinin isolectins A and D. Smith JJ, Raikhel NV. Plant Mol Biol. 1989 Nov . 13(5):601-3. 10.1007/BF00027321 PubMed 2491677
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