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pCDH-AtAFB2-mTagBFP2
(Plasmid #179889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH
  • Backbone manufacturer
    Systembio
  • Backbone size w/o insert (bp) 6390
  • Total vector size (bp) 8840
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral
  • Selectable markers
    mTagBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtAFB2
  • Alt name
    AFB2
  • Alt name
    AT3G26810
  • Alt name
    auxin signaling F-box 2
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    2450
  • GenBank ID
    MP787830.1
  • Entrez Gene
    AFB2 (a.k.a. AT3G26810, auxin signaling F-box 2)
  • Promoter EF1
  • Tag / Fusion Protein
    • mTagBFP2 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This lentiviral plasmids delivers AtAFB2 (auxin-inducible degron system) fused to mTagBFP2. In combination with TetOn3G can be used to generate DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation.

Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-AtAFB2-mTagBFP2 was a gift from Patrick Caswell (Addgene plasmid # 179889 ; http://n2t.net/addgene:179889 ; RRID:Addgene_179889)

  • For your References section:

    On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members. Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT. Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651. 10.7554/eLife.76651 PubMed 35708998
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