pCDH-EF1a-mTagBFP-T2A-TetOn3G
(Plasmid #179888)

• Purpose: Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)
• Depositing Lab: Patrick Caswell
• Publication: Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.

Backbone

• Vector backbone: pCDH
• Backbone manufacturer: Systembio
• Backbone size w/o insert (bp): 6370
• Total vector size (bp): 7911
• Vector type: Mammalian Expression, Bacterial Expression, Lentiviral
• Selectable markers: mTagBFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TetOn3G
• Promoter: EF1a
• Tag / Fusion Protein:
  • mTagBFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTCCACGCTTTGCCTGACCCTGCTT

Resource Information

• Supplemental Documents:
  • pcdh-ef1a-tagbfp-t2a-teton3g.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control)
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.

Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.