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pCOA-Leu-FvPPT1-fsr1
(Plasmid #179868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pESC-LEU
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 8660
  • Total vector size (bp) 16436
  • Modifications to backbone
    Added broad host-range origin of replication ARS68 from Yarrowia lipolytica. Subtituted GAL1/10 bidirectional promoter for Yarrowia lipolytica EXP1 and TEF-1α.
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PPT1
  • Alt name
    FvPPT1
  • Alt name
    PPTase
  • Species
    Fusarium verticillioides
  • Insert Size (bp)
    879
  • Mutation
    Codon optimized for expression in Saccharomyces cerevisiae
  • GenBank ID
    FVEG_01894 XP_018744954
  • Promoter Yarrowia lipolytica EXP1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    fsr1
  • Alt name
    PKS3
  • Species
    Fusarium vanettenii 77-13-4
  • Insert Size (bp)
    6897
  • Mutation
    Codon optimized for expression in Saccharomyces cerevisiae
  • GenBank ID
    NECHADRAFT_101778 XP_003039929
  • Promoter Yarrowia lipolytica TEF-1α

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCOA-Leu-FvPPT1-fsr1 was a gift from Jens Laurids Sørensen (Addgene plasmid # 179868 ; http://n2t.net/addgene:179868 ; RRID:Addgene_179868)

  • For your References section:

    Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students. Bejenari M, Nielsen L, Spedtsberg EML, Nielsen MR, Pedersen TB, Sorensen JL. J Microbiol Biol Educ. 2023 Nov 27;24(3):e00242-22. doi: 10.1128/jmbe.00242-22. eCollection 2023 Dec. 10.1128/jmbe.00242-22 PubMed 38108002
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