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Addgene

pUC57-fsr1
(Plasmid #179866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 9607
  • Modifications to backbone
    -
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusarubin polyketide synthase
  • Alt name
    fsr1
  • Alt name
    PKS3
  • Species
    Fusarium vanettenii 77-13-4
  • Insert Size (bp)
    6897
  • Mutation
    Codon optimized for expression in Saccharomyces cerevisiae
  • GenBank ID
    NECHADRAFT_101778 EEU34216.1

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene insert was synthesized by Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-fsr1 was a gift from Jens Laurids Sørensen (Addgene plasmid # 179866 ; http://n2t.net/addgene:179866 ; RRID:Addgene_179866)

  • For your References section:

    Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students. Bejenari M, Nielsen L, Spedtsberg EML, Nielsen MR, Pedersen TB, Sorensen JL. J Microbiol Biol Educ. 2023 Nov 27;24(3):e00242-22. doi: 10.1128/jmbe.00242-22. eCollection 2023 Dec. 10.1128/jmbe.00242-22 PubMed 38108002
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