Skip to main content
Addgene

CMV-T7-hOsa1
(Plasmid #17986)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCGT
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Osa1
  • Alt name
    BAF250a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6400
  • Mutation
    Missing 5' end
  • GenBank ID
    AF521670
  • Entrez Gene
    ARID1A (a.k.a. B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1)
  • Tag / Fusion Protein
    • T7 epitope (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site XhoI-SalI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-T7-hOsa1 was a gift from Naoko Tanese (Addgene plasmid # 17986 ; http://n2t.net/addgene:17986 ; RRID:Addgene_17986)

  • For your References section:

    Largest subunits of the human SWI/SNF chromatin-remodeling complex promote transcriptional activation by steroid hormone receptors. Inoue H, Furukawa T, Giannakopoulos S, Zhou S, King DS, Tanese N. J Biol Chem. 2002 Nov 1. 277(44):41674-85. 10.1074/jbc.M205961200 PubMed 12200431
Related items:
Commonly requested with: