Skip to main content
Addgene

Multiplex-CRISPR-miR-200FAM-T2A-GFP
(Plasmid #179856)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLV hUbC-Cas9-T2A-GFP
  • Backbone manufacturer
    Charles Gersbach (Addgene plasmid # 53190)
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 15000
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c )
  • gRNA/shRNA sequence
    gRNA sequence details listed in Supplementary Information Table S4 of the Article and below.
  • Species
    H. sapiens (human)
  • GenBank ID
    miR-200 family: Homo sapiens miR-200b, LM608401.1, Homo sapiens miR-429, LM379507.1, Homo sapiens miR-141: LM379050.1 Homo sapiens miR-200c: LM608608.1, Homo sapiens miR-200a: LM608664.1
  • Entrez Gene
    MIR141 (a.k.a. MIRN141, mir-141)
  • Entrez Gene
    MIR200B (a.k.a. MIRN200B, mir-200b)
  • Entrez Gene
    MIR429 (a.k.a. MIRN429, hsa-mir-429, mir-429)
  • Entrez Gene
    MIR200C (a.k.a. MIRN200C, mir-200c)
  • Entrez Gene
    MIR200A (a.k.a. MIRN200A, mir-200a)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Oligonucleotides for cloning protospacers:
h200b-mU6-O1 TTGTTTGGCCATCTTACTGGGCAGCAT
h200b-mU6-O2 AAACATGCTGCCCAGTAAGATGGCCAA
h429-hU6-O1 CACCGGCCCTCTGTCTAATACTGTC
h429-hU6-O2 AAACGACAGTATTAGACAGAGGGCC
h200c-7SK-O1 CCTCGTCGTCTTACCCAGCAGTGTT
h200c-7SK-O2 AAACAACACTGCTGGGTAAGACGAC
h141-H1-O1 TCCCATCCAACACTGTACTGGAAGA
h141-H1-O2 AAACTCTTCCAGTACAGTGTTGGAT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Multiplex-CRISPR-miR-200FAM-T2A-GFP was a gift from Brendan Jenkins (Addgene plasmid # 179856 ; http://n2t.net/addgene:179856 ; RRID:Addgene_179856)

  • For your References section:

    Complete loss of miR-200 family induces EMT associated cellular senescence in gastric cancer. Yu L, Cao C, Li X, Zhang M, Gu Q, Gao H, Balic JJ, Xu D, Zhang L, Ying L, Xu D, Yang Y, Wu D, He B, Jenkins BJ, Liu Y, Li J. Oncogene. 2021 Oct 19. pii: 10.1038/s41388-021-02067-y. doi: 10.1038/s41388-021-02067-y. 10.1038/s41388-021-02067-y PubMed 34667277
Related items:
Commonly requested with: