Moffat Lab - Barcode Libraries
(Pooled Libraries #179851, #179852)

• Purpose:

  This lentiviral barcoding library consists of 10⁶ unique barcodes. Each barcode contains a 12-bp degenerate region followed by two static bases (either C or G) and a unique 4-bp library code.

  The library was constructed by cloning the barcodes into a third generation lentiviral vector containing both a Puromycin selection cassette and the ZsGreen fluorescent marker.

  Cellular barcoding can be used to trace clonal dynamics of individual cells using stable, genetically distinct sequences inherited by all progeny.

• Vector Backbone:

  pLJM1

• Depositing Labs: Jason Moffat
• Publication: Rehman et al. Cell. 2021 Jan 7;184(1):226-242.e21. doi: 10.1016/j.cell.2020.11.018.

Library Details

• Number of barcodes: >10⁶
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼40 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (DOCX, 17 KB)
  • Library Sequencing Protocol (DOCX, 134 KB)
  • Deconvolution Algorithm (DOCX, 15 KB)
• Scripts:
  • Barcode Deconvolution Script (PL, 3.5 KB)
• Depositor Data:
  • Barcode Index Primers (XLSX, 15 KB)
  • Library Complexity (PDF, 238 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Both are high-diversity libraries identifiable by a unique 4-bp library code: BCL0 uses ACGT; BLC1 uses CCAA. Either library can be used for an individual experiment.

The libraries have also been used and published in Chang et al. 2021 (preprint available on bioRxiv: doi.org/10.1101/2020.08.28.273029 ).