XLone-Puro CCND2-T2A-luciferase-P2A-eGFP
(Plasmid #179843)

• Purpose: Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP
• Depositing Lab: Xiaoping Bao
• Publication: Joshi et al Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 10.3390/cells11060951.

Backbone

• Vector backbone: Addgene #140027
• Backbone size w/o insert (bp): 5760
• Total vector size (bp): 9146
• Vector type: Mammalian Expression, Luciferase, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CCND2
• Alt name: KIAK0002
• Alt name: MPPH3
• Species: H. sapiens (human)
• Insert Size (bp): 867
• Entrez Gene: CCND2 (a.k.a. KIAK0002, MPPH3)
• Promoter: TRE3G
• Tags / Fusion Proteins:
  • luciferase (C terminal on insert)
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry