AAVS1-Puro cTnT CCND2-T2A-ChloC
(Plasmid #179841)

• Purpose: donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells
• Depositing Lab: Xiaoping Bao
• Publication: Joshi et al Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 10.3390/cells11060951.

Backbone

• Vector backbone: Addgene #136935
• Backbone size w/o insert (bp): 9222
• Total vector size (bp): 11129
• Vector type: Mammalian Expression, CRISPR, TALEN ; Donor plasmid
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CCND2
• Alt name: KIAK0002
• Alt name: MPPH3
• Species: H. sapiens (human)
• Insert Size (bp): 867
• Entrez Gene: CCND2 (a.k.a. KIAK0002, MPPH3)
• Promoter: Troponin T promoter
• Tag / Fusion Protein:
  • chloride-conducting ChR (ChloC) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains E83Q and E101S mutations in CCND2. These mutations are not known to affect the plasmid's functions.