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AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
(Plasmid #179840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene #136935
  • Backbone size w/o insert (bp) 9222
  • Total vector size (bp) 11939
  • Vector type
    Mammalian Expression, CRISPR, TALEN ; Donor plasmid
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCND2
  • Alt name
    KIAK0002
  • Alt name
    MPPH3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    867
  • Entrez Gene
    CCND2 (a.k.a. KIAK0002, MPPH3)
  • Promoter Troponin T promoter
  • Tags / Fusion Proteins
    • chloride-conducting ChR (ChloC) (C terminal on insert)
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains E83Q and E101S mutations in CCND2. These mutations are not known to affect the plasmid's functions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP was a gift from Xiaoping Bao (Addgene plasmid # 179840 ; http://n2t.net/addgene:179840 ; RRID:Addgene_179840)

  • For your References section:

    Optogenetic Control of Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study. Joshi J, Xu B, Rubart M, Chang Y, Bao X, Chaliki HP, Scott LR, Zhu W. Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 10.3390/cells11060951. 10.3390/cells11060951 PubMed 35326403
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