pAXY0002
(Plasmid
#179835)
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Purpose(Empty Backbone) binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179835 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAMBIA1305.1
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Backbone manufacturerCAMBIA
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Modifications to backboneThe Hygromycin resistance gene is replaced by a Kanamycin resistance gene.
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Vector typePlant Expression ; binary vector for plant transformation
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAXY0002 was a gift from Xiaohan Yang (Addgene plasmid # 179835 ; http://n2t.net/addgene:179835 ; RRID:Addgene_179835) -
For your References section:
Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants. Yuan G, Lu H, Tang D, Hassan MM, Li Y, Chen JG, Tuskan GA, Yang X. Hortic Res. 2021 Nov 1;8(1):234. doi: 10.1038/s41438-021-00663-3. 10.1038/s41438-021-00663-3 PubMed 34719678
