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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonena
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTAF4
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Alt nameTAF130
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Alt namehTAFII130
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SpeciesH. sapiens (human)
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Insert Size (bp)4000
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Mutationmissing 5' end
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GenBank IDU75308
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Entrez GeneTAF4 (a.k.a. MRD73, TAF(II)130, TAF(II)135, TAF2C, TAF2C1, TAF4A, TAFII-130, TAFII-135, TAFII130, TAFII135)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nde I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-HA-hTAF4 was a gift from Naoko Tanese (Addgene plasmid # 17983 ; http://n2t.net/addgene:17983 ; RRID:Addgene_17983) -
For your References section:
Molecular cloning and analysis of two subunits of the human TFIID complex: hTAFII130 and hTAFII100. Tanese N, Saluja D, Vassallo MF, Chen JL, Admon A. Proc Natl Acad Sci U S A. 1996 Nov 26. 93(24):13611-6. 10.1073/pnas.93.24.13611 PubMed 8942982
Map uploaded by the depositor.
