Skip to main content
Addgene

SPLINTR GFP v1 backbone
(Plasmid #179777)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    MS2-P65-HSF1_GFP plasmid (Addgene #61423)
  • Backbone manufacturer
    Feng Zhang
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BspEI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SPLINTR GFP v1 backbone was a gift from Mark Dawson (Addgene plasmid # 179777 ; http://n2t.net/addgene:179777 ; RRID:Addgene_179777)

  • For your References section:

    Non-genetic determinants of malignant clonal fitness at single-cell resolution. Fennell KA, Vassiliadis D, Lam EYN, Martelotto LG, Balic JJ, Hollizeck S, Weber TS, Semple T, Wang Q, Miles DC, MacPherson L, Chan YC, Guirguis AA, Kats LM, Wong ES, Dawson SJ, Naik SH, Dawson MA. Nature. 2022 Jan;601(7891):125-131. doi: 10.1038/s41586-021-04206-7. Epub 2021 Dec 8. 10.1038/s41586-021-04206-7 PubMed 34880496
Related items:
Commonly requested with: