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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMS2
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Vector typemycobacteria expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionshygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria. This plasmid is high copy number in E. coli and low copy number in mycobacteria.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepmyc1tetO-gfp, tetR(B)
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Alt nameGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379.
pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUV15tetORm was a gift from Sabine Ehrt (Addgene plasmid # 17975 ; http://n2t.net/addgene:17975 ; RRID:Addgene_17975) -
For your References section:
Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein M, Ehrt S, Schnappinger D. J Bacteriol. 2007 Jul . 189(13):4614-23. 10.1128/JB.00216-07 PubMed 17483222
Map uploaded by the depositor.
