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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMS2
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Vector typemycobacteria expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThis plasmid is high copy number in E. coli and low copy number in mycobacteria.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametetR(B)r1.7
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Alt namereverse tetR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281.
Kanamycin resistant derivative of pTE-4S0X.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTEK-4S-0X was a gift from Sabine Ehrt (Addgene plasmid # 17974 ; http://n2t.net/addgene:17974 ; RRID:Addgene_17974) -
For your References section:
Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein M, Ehrt S, Schnappinger D. J Bacteriol. 2007 Jul . 189(13):4614-23. 10.1128/JB.00216-07 PubMed 17483222
Map uploaded by the depositor.
