pTE-4S-0X
(Plasmid #17973)

• Depositing Lab: Sabine Ehrt
• Publication: Guo et al J Bacteriol. 2007 Jul . 189(13):4614-23.

Backbone

• Vector backbone: pMS2
• Vector type: mycobacteria expression

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria). This plasmid is high copy number in E. coli and low copy number in mycobacteria.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tetR(B)r1.7
• Alt name: reverse tetR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281.

Derivative of pMS2 with tetR r1.7 expressed by Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.