pME1mL1
(Plasmid #17971)

• Depositing Lab: Sabine Ehrt
• Publication: Guo et al J Bacteriol. 2007 Jul . 189(13):4614-23.

Backbone

• Vector backbone: pMS2
• Vector type: mycobacteria expression

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria, This plasmid is high copy number in E. coli and low copy number in mycobacteria.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pmyc1tetO-lacZ, tetR(B)
• Alt name: LacZ

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.

Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.