pmCherry-HP1alpha
(Plasmid #179703)

• Purpose: visualization of centromere heterochromatin
• Depositing Lab: Kenji Sugimoto
• Publication: Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7):1255-1262. doi: 10.1080/09168451.2019.1591265. Epub 2019 Mar 24.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: heterochromatin protein alpha
• Species: H. sapiens (human)
• Entrez Gene: CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NheI-BglII-digested PCR products pREST-B-mCherry into the corresponding sites of the vector backbone of pEGFP-C1