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Addgene

pDsRed-HP1alpha
(Plasmid #179702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDsRed-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    heterochromatin protein alpha
  • Species
    H. sapiens (human)
  • Entrez Gene
    CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
  • Tag / Fusion Protein
    • dsRed (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDsRed-HP1alpha was a gift from Kenji Sugimoto (Addgene plasmid # 179702 ; http://n2t.net/addgene:179702 ; RRID:Addgene_179702)

  • For your References section:

    Live-cell imaging of HP1alpha throughout the cell cycle of mouse C3H10T1/2 cells and rhythmical flickering of heterochromatin dots in interphase. Nakagawa C, Kawakita A, Fukada T, Sugimoto K. Biosci Biotechnol Biochem. 2014;78(4):556-64. doi: 10.1080/09168451.2014.893184. Epub 2014 May 9. 10.1080/09168451.2014.893184 PubMed 25036950
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