Skip to main content
Addgene

pmPlum-histone H3
(Plasmid #179700)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179700 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Hist1h3h (a.k.a. H3-291)
  • Tag / Fusion Protein
    • mPlum (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tachibana et al. 2001 doi: 10.1074/jbc.m101914200

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.hilarispublisher.com/open-access/molecular-behavior-of-cenpa-and-pcna-throughout-the-cell-cycle-inliving-human-ht1080-cells-2168-9547-1000162.pdf to access the publication.

Plasmid pmPlum-C1 was constructed by inserting the amplified DNA fragment encoding mPlum of pBAD-mPlum.

For more information, please also see Tanaka et al. (2017) bioimages. https://doi.org/10.11169/bioimages.25.7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmPlum-histone H3 was a gift from Kenji Sugimoto (Addgene plasmid # 179700 ; http://n2t.net/addgene:179700 ; RRID:Addgene_179700)

  • For your References section:

    Molecular Behavior of CENP-A and PCNA throughout the Cell Cycle in Living Human HT-1080 Cells. Nakagawa C, Senda-Murata K, Kawakita-Yamaguchi A, Fukada T, Sugimoto K. Mol Biol 2016, 5:2 10.4172/2168-9547.1000160
Related items: