Skip to main content
Addgene

pECFP-histone H3
(Plasmid #179698)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pECFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Hist1h3h (a.k.a. H3-291)
  • Tag / Fusion Protein
    • ECFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tachibana et al. 2001 doi: 10.1074/jbc.m101914200

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EcoRI-XhoI fragment of pZErO-histone H3 (Tachibana et al. 2001 https://doi.org/10.1074/jbc.m101914200)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECFP-histone H3 was a gift from Kenji Sugimoto (Addgene plasmid # 179698 ; http://n2t.net/addgene:179698 ; RRID:Addgene_179698)

  • For your References section:

    Molecular dynamics of Aurora-A kinase in living mitotic cells simultaneously visualized with histone H3 and nuclear membrane protein importinalpha. Sugimoto K, Urano T, Zushi H, Inoue K, Tasaka H, Tachibana M, Dotsu M. Cell Struct Funct. 2002 Dec;27(6):457-67. PubMed 12576638
Related items:
Commonly requested with: