pH6-MBP-TEV-BirA
(Plasmid #179694)

• Purpose: Expresses His6 tagged MBP-BirA fusion protein
• Depositing Lab: Mark Arbing
• Publication: Bacterial Expression Plasmid for H6-MBP-BirA (unpublished)

Backbone

• Vector backbone: pMAL-C2
• Backbone manufacturer: New England Biolabs
• Total vector size (bp): 7605
• Modifications to backbone: His6-thrombin site added at N-terminus of MBP.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BirA
• Species: Escherichia coli (strain K12)
• GenBank ID: U00006
• Tag / Fusion Protein:
  • His6-MBP (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• Supplemental Documents:
  • ph6-mbp-bira.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Express MBP-BirA fusion protein overnight at 18C. Lysis buffer: 50 mM Tris pH 8, 300 mM NaCl. Size exclusion buffer: 50 mM Tris pH 8.0, 150 mM NaCl.