pMC1s
(Plasmid #17969)

• Depositing Lab: Sabine Ehrt
• Publication: Guo et al J Bacteriol. 2007 Jul . 189(13):4614-23.

Backbone

• Vector backbone: pMV306
• Backbone size w/o insert (bp): 4500
• Vector type: mycobacteria expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: This plasmid is low copy number in E. coli and integrative in mycobacteria.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: tetR (B)
• Alt name: tetracycline repressor
• Alt name: wild-type tetR
• Insert Size (bp): 600

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

tetR is not codon-usage adapted for mycobacteria. tetR is expressed from a strong mycobacterial promoter (psmyc = puv15).

This is a derivative of pMV306K, which is a single-copy-integrating plasmid which inserts into L5 attB. Also see Pubmed ID 15687379.