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Addgene

pBM13g-sd4U
(Plasmid #179689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    [sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)
  • Species
    Synthetic
  • Mutation
    S1330I A1341V N1342S E1370K T1380I

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

araC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBM13g-sd4U was a gift from David Liu (Addgene plasmid # 179689 ; http://n2t.net/addgene:179689 ; RRID:Addgene_179689)

  • For your References section:

    CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA. Mok BY, Kotrys AV, Raguram A, Huang TP, Mootha VK, Liu DR. Nat Biotechnol. 2022 Sep;40(9):1378-1387. doi: 10.1038/s41587-022-01256-8. Epub 2022 Apr 4. 10.1038/s41587-022-01256-8 PubMed 35379961
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