ND4.2-Left TALE-G1397-N-DddA11-mCherry
(Plasmid
#179682)
-
PurposeExpression of base editor in mammalian cells and for cell sorting
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 179682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
-
SpeciesSynthetic
-
MutationS1330I A1341V N1342S E1370K T1380I
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ND4.2-Left TALE-G1397-N-DddA11-mCherry was a gift from David Liu (Addgene plasmid # 179682 ; http://n2t.net/addgene:179682 ; RRID:Addgene_179682) -
For your References section:
CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA. Mok BY, Kotrys AV, Raguram A, Huang TP, Mootha VK, Liu DR. Nat Biotechnol. 2022 Sep;40(9):1378-1387. doi: 10.1038/s41587-022-01256-8. Epub 2022 Apr 4. 10.1038/s41587-022-01256-8 PubMed 35379961